To further confirm their findings, Drs. Rife and Kendall next examined 18-hour-old cultures which had been inoculated into K Medium and incubated at 37 degrees C., since it is just at this stage of growth in this medium and at this temperature that the cultures became filterable. And, just as had been anticipated, ordinary dark-field examination revealed unchanged, long, actively motile bacilli; bacilli having granules within their substance; and free swimming, actively motile granules; while under the Rife microscope were demonstrated the same long, unchanged, almost colorless bacilli; bacilli, practically colorless, inside and at one end of which was a turquoise granule resembling the filterable forms of the typhoid bacillus; and free swimming, small, oval, actively motile, turquoise-blue granules. By transplanting the cultures of the filter-passing organisms or virus in to a broth, they were seen to change over again into their original rod-like forms.
At the same time that these findings of Drs. Rife and Kendall were confirmed by Dr. Edward C. Rosenow, of the Mayo Foundation, the magnification with accompanying resolution of 8,000 diameters of the Rife microscope, operated by Dr. Rife, was checked against a dark-field oil-immersion scope operated by Dr. Kendall and an ordinary 2-mm. oil-immersion objective, X 10 ocular, Zeiss scope operated by Dr. Rosenow at a magnification of 900 diameters. Examinations of gram- and safrinin-stained films of cultures of Bacillus typhosus, gram- and safrinin-stained films of cultures of the streptococcus from poliomyelitis, and stained films of blood and of the sediment of the spinal fluid from a case of acute poliomyelitis were made with the result that bacilli, streptococci, erythrocytes, polymorphonuclear leukocytes, and lymphocytes measuring nine times the diameter of the same specimens observed under the Zeiss scope at a magnification and resolution of 900 diameters, were revealed with unusual clarity. Seen under the dark-field microscope were moving bodies presumed to be the filterable turquoise-blue bodies of the typhoid bacillus which, as Dr. Rosenow has declared in his report (Observations on filter-passing forms of Eberthella typhi – Bacillus typhosus – and of the streptococcus from poliomyelitis, Proc. Staff Meetings Mayo Clinic, July 13, 1932), were so “unmistakably demonstrated” with the Rife microscope, while under the Zeiss scope stained and hanging-drop preparations of clouded filtrate cultures were found to be uniformly negative. With the Rife microscope also were demonstrated brownish-grey cocci and diplococci in hanging drop preparations of the filtrates of streptococcus from poliomyelitis. These cocci and diplococci, similar in size and shape to those seen in the cultures although of more uniform intensity, and the characteristic of the medium in which they had been cultivated, were surrounded by a clear halo about twice the width of that at the margins of the debris and of the Bacillus typhosus. Stained films of filtrates and filtrate sediments examined under the Zeiss microscope, and hanging-drop, dark-field preparations revealed no organisms, however. Brownish-grey cocci and diplococci of the exact same size and density as those observed in the filtrates of the streptococcus cultures were also revealed in hanging-drop preparations of the virus of poliomyelitis under the Rife microscope, while no organisms at all could be seen in either the stained films of filtrates and filtrate sediments examined with the Zeiss scope or in hanging drop preparations examined by means of the dark-field. Again using the Rife microscope at a magnification of 8,000 diameters, numerous nonmotile cocci and diplococci of the streptococcus and poliomyelitis viruses, they were shown to be of fairly even density, size, and form and surrounded by a halo. Again, both the dark-field and Zeiss scopes failed to reveal any organisms, and none of the three microscopes disclosed the presence of such diplococci in hanging-drop preparations of the filtrate of a normal rabbit brain. Dr. Rosenow has since revealed these organisms with the ordinary microscope at a magnification of 1,000 diameters by means of his special staining method and with the electron microscope at a magnification of 12,000 diameters. Dr. Rosenow has expressed the opinion that the inability to see these and other similarly revealed organisms is due, not necessarily to the minuteness of the organisms, but rather to the fact that they are of a nonstaining, hyaline structure. Results with the Rife microscopes, he thinks, are due to the “ingenious methods employed rather than to excessively high magnification.” He declared also, in the report mentioned previously, that “Examination under the Rife microscope of specimens containing objects visible with the ordinary microscope, leaves no doubt of the accurate visualization of objects or particulate matter by direct observation at the extremely high magnification obtained with this instrument.”
Exceedingly high powers of magnification with accompanying high powers of resolution may be realized with all of the Rife microscopes, one of which, having magnification and resolution up to 18,000 diameters, is now being used at the British School of Tropical Medicine in England. In the recent demonstration of another of the smaller Rife scopes (May 16, 1942) before a group of doctors including Dr. J.H. Renner, of Santa Barbara, Calif.; Dr. Roger A. Schmidt, of San Francisco, Calif.; Dr. Lois Bronson Slade, of Alameda, Calif.; Dr. Lucille B. Larkin, of Bellingham, Wash.; Dr. E.F. Larkin, of Bellingham, Wash.; and Dr. W.J. Gier, of San Diego, Calif., a Zeiss ruled grading was examined first under an ordinary commercial microscope equipped with a 1.8 high dry lens and X 10 ocular, and then under the Rife microscope. Whereas 50 lines were revealed with the commercial instrument and considerable aberration, both chromatic and spherical noted, only 5 lines were seen with the Rife scope, these 5 lines being so highly magnified that they occupied the entire field, without any aberration whatsoever being apparent. Dr. Renner, in a discussion of his observations, stated that ” The entire field to its very edge and across the center had a uniform clearness that was not true in the conventional instrument.” Following the examination of the grading, an ordinary unstained blood film was observed under the same two microscopes. In this instance, 100 cells were seen to spread throughout the field of the commercial instrument while but 10 cells filled the field of the Rife scope.
The universal microscope, of course, is the most powerful Rife scope, possessing a resolution of 31,00 diameters and a magnification of 60,000 diameters. With this it is possible to view the interior of the “pin-point” cells, those cells situated between the normal tissue cells and just visible under the ordinary microscope, and to observe the smaller cells which compose the interior of these pin-point cells. When one of these smaller cells is magnified, still smaller cells are seen within its structure. And when one of the still smaller cells, in its turn, is magnified, it, too, is seen to be composed of smaller cells. Each of the 16 times this process of magnification and resolution can be repeated, it is demonstrated that there are smaller cells within the smaller cells, a fact which amply testifies as to the magnification and resolving power obtainable with the universal microscope.
More than 20,000 laboratory cultures of carcinoma were grown and studied over a period of 7 years by Dr. Rife and his assistants in what, at the time, appeared to be fruitless effort to isolate the filter-passing form, or virus, which Dr. Rife believed to be present in this condition. Then, in 1932, the reactions in growth of bacterial cultures to light from rare gases was observed, indicating a new approach to the problem. Accordingly, blocks of tissue one-half centimeter square, taken from an unulcerated breast carcinoma, were placed in triple-sterilized K Medium and these cultures incubated at 37 degrees C> When no results were forthcoming, the culture tubes were placed in a circular glass loop filled with argon gas to a pressure of 14 millimeters, and a current of 5,000 volts applied for 24 hours, after which the tubes were placed in a 2-inch water vacuum and incubated at 37 degrees C. for 24 hours. Using a specially designed 1.12 dry lens, equal in amplitude of magnification to the 2-mm. apochromatic oil-immersion lens, the cultures were then examined under the universal microscope, at a magnification of 10,000 diameters, where very much animated, purplish-red, filterable forms, measuring less than one-twentieth of a micron in dimension, were observed. Carried through 14 transplants from K Medium to K Medium, this B.X. virus remained constant; inoculated into 426 Albino rats, tumors “with all the true pathology of neoplastic tissue” were developed. Experiments conducted in the Rife Laboratories have established the fact that these characteristic diplococci are found in the blood monocytes in 92 percent of all cases of neoplastic diseases. It has also been demonstrated that the virus of cancer, like the viruses of other diseases, can be easily changed from one form to another by means of altering the media upon which it is grown. With the first change in media, the B.X. virus becomes considerably enlarged although its purplish-red color remains unchanged. Observation of the organism with an ordinary microscope is made possible by a second alteration of the media. A third change is undergone upon asparagus base media where the B.X. virus is transformed from its filterable state into cryptomyces pleomorphia fungi, these fungi being identical morphologically both macroscopically and microscopically to that of the orchid and of the mushroom. And yet a fourth change may be said to take place when this cryptomyces pleomorphia, permitted to stand as a stock culture for the period of metastasis, becomes the well-known mahogany-colored Bacillus coli.